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Test Code HCVQR HCV RNA BY PCR, QUANTITATIVE

Methodology

Polymerase Chain Reaction (PCR)

Performing Laboratory

West Virginia University Hospital, Inc.

Specimen Requirements

Collect: Two lavender top tubes

Minimum Collection Volume: 7 mL blood

Stability: For plasma separated from cells within 24 hours of collection

   Refrigerated: 6 days (plasma separated from red blood cells)     

   Frozen (-70° C): 12 weeks (preferred storage)

Required for Testing: 2.5 mL plasma

 

Reject Due To: Specimens collected in heparin tubes. Heparin inhibits the PCR reaction included in this assay.

Reference Values

Target not Detected

Day(s) Test Set Up

Monday-Friday (varies)

Test Classification and CPT Coding

87522

UML Lab Processing Instructions

Transport Temperature: Frozen

UML Test Code: HCVQR

Interpretive Information

EDTA plasma specimens are analyzed by the Roche COBAS 6800 assay. This is a FDA approved assay.

WVUH Lab Processing Instructions

Plasma must be separated from cells within 24 hours of collection. Aliquot 2.5 mL of plasma and place aliquot in the -20 freezer.